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Fig. 8 | Cell & Bioscience

Fig. 8

From: DNA methyltransferase 1 knockdown reverses PTEN and VDR by mediating demethylation of promoter and protects against renal injuries in hepatitis B virus-associated glomerulonephritis

Fig. 8

The transcription factor SNAI1 represses VDR in HBx-podocytes. A Luciferase activities of podocyte lysates subject to basic vector (Vector) or pcDNA3.1/Flag-Snai1 (SNAI1) transfection with pGL4.10-VDR wild-type promoter (VDR-WT) or VDR mutant promoter (Mut1 and Mut2). Renilla activity was used to normalize firefly activity. B HBx-podocytes were transfected with the VDR reporter plasmid and a Renilla luciferase reporter, which were subsequently treated with or without SNAI1 knockdown. Renilla activity was adopted for normalizing firefly activity. Results are expressed as mean ± SD of 3 separate assays. *P < 0.05; **P < 0.01

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Cell & Bioscience

ISSN: 2045-3701

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