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Fig. 7 | Cell & Bioscience

Fig. 7

From: DNA methyltransferase 1 knockdown reverses PTEN and VDR by mediating demethylation of promoter and protects against renal injuries in hepatitis B virus-associated glomerulonephritis

Fig. 7

DNMT1 regulated VDR methylation during kidney injuries in HBx-podocytes. A The localization and expression of DNMT1 and VDR in HBx-podocytes were determined by immunofluorescence. B DNMT1 and SNAI1 levels and localization within HBx-podocytes detected through immunofluorescence. Scale bar: 10 μm. C Western blotting for p-P65, P65, p-IκBα, and IκBα in HBx-podocytes treated with or without inhibition of DNMT1 or VDR. D and E Concentrations of TNF-α and IL-6 in the supernatant of HBx-podocytes with or without transfecting siDNMT1 or siVDR were evaluated by ELISA. F Western blotting for VDR, Podocin, Nephrin, ZO1, Vimentin, and Fibronectin in HBx-podocytes treated with or without inhibition of DNMT1 or VDR. G Representative BSP analysis for the DNA methylation status of the VDR promoter region (28 CpG dinucleotides) in human podocytes and HBx-podocytes transfected with siNC or siDNMT1. Methylated CpGs are indicated as black dots; unmethylated CpGs are indicated as empty dots. Results are expressed as mean ± SD of 3 separate assays. *P < 0.05; **P < 0.01

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Cell & Bioscience

ISSN: 2045-3701

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