Fig. 4

RNA-sequencing after DNMT1 knockdown in HBx-overexpressing renal cells. A PCA in HK-2 cells and siNC- or siDNMT1-transfected HBx-HK-2 cells (three replicates). B KEGG pathway analyses for identifying potential pathways in HK-2 cells and siNC- or siDNMT1-transfected HBx-HK-2 cells. C Differentially expressed genes (79 upregulated and 97 downregulated) identified in the volcano plot for siNC- or siDNMT1-transfected HBx-HK-2 cells. D Heatmap showing 25 differentially expressed genes in siNC- or siDNMT1-transfected HBx-HK-2 cells. E RT-qPCR and western blotting were performed to confirm the altered mRNA and protein expression of PTEN after knocking down DNMT1. F PCA of podocytes and siNC- or siDNMT1-transfected HBx-podocytes with three replicates. G KEGG pathway analyses for potential pathways in podocytes and siNC- or siDNMT1-transfected HBx-podocytes. H Volcano plot revealed differentially expressed genes (291 upregulated and 131 downregulated) in siNC- or siDNMT1-transfected HBx-podocytes. I Heatmap showing 25 differentially expressed genes in siNC- or siDNMT1-transfected podocytes. J RT-qPCR and western blotting were performed to confirm the altered mRNA and protein expression of SNAI1 and VDR after knocking down DNMT1. Data are expressed as mean ± SD of 3 separate assays. *P < 0.05; **P < 0.01