Fig. 1

DNMT1 upregulation among HBV-GN patients and HBx-transfected renal cells. A DNMT1 expression was determined using immunohistochemistry. B and C HK-2 cells and human podocytes transfected with empty vector lentivirus (NC) or HBx-expressing lentivirus (HBx) and without transfection (Cont) were examined for HBx expression by western blotting and RT-qPCR. D The expression of DNMT1, DNMT3a, and DNMT3b was measured in HK-2 cells and human podocytes using western blotting. E DNMT1 activity was evaluated using the DNMT1 assay kit. Data are expressed as mean ± SD of 3 separate assays. *P < 0.05; **P < 0.01