Fig. 8

C3aR blockade inhibited synaptic pruning in the prefrontal cortex of CUMS mice. Representative photographs of Iba1(red)/PSD95(green)/DAPI(blue) and the 3D reconstruction (A). Representative photographs of Iba1(red)/synaptophysin (green)/DAPI(blue) and the 3D reconstruction (B). C3aR blockade restored the microglial morphology (C-H), prevented the engulfment of PSD95 by microglia according to area (I) and volume (J) analysis, and prevented the engulfment of synaptophysin by microglia according to area (K) and volume (L) analysis. Representative photographs of dendritic spines by Golgi staining (M). C3aR blockade enhanced dendritic spines in CUMS-induced mice (N). Data were obtained from 5 samples per group. ^##P < 0.01 versus the Control-vehicle group. ^*P < 0.05 and ^**P < 0.01 versus the CUMS-vehicle group