Fig. 5

H3K27ac mediates SS18/BAFs relocation and PST. A The expression of representative downstream genes of Jun were detected by real-time qPCR at 6 h during PST with 30 μM Brg1 bromodomain inhibitor, PFI-3 (P) and 30 μM Brd7/Brd9 bromodomain inhibitor, BI-9564 (B) treatment, and combined P + B. Data are mean ± s.d., two tailed, unpaired t-test; n = 4 independent experiments, *P < 0.05, **P < 0.01, ***P < 0.001. B ChIP-seq heatmap showing the SS18 dynamic changes and SS18 ChIP-seq from P + B and DMSO during PST. Loci of chromatin were arranged into three groups depending upon the fold change of strength of SS18 binding peaks between 0 and 8 h. Fold change 2. Down stands for 8 h compare to 0 h < 2, number of peaks = 1685, up stands for 8 h compare to 0 h > 2, number of peaks = 6875, the others peaks stand permanent, number of peaks = 6422. C Selected genomic views of ChIP data and RNA-seq data are shown for the indicated Down, Up, Permanent groups. Loci of indicated groups are marked with gray boxes. D Representative images for 48 h in 2i/LIF medium after c-Jun induction with different treatment, DMSO(Dox 0 h) DMSO(Dox 6 h), PFI-3(30 μM, Dox 6 h), BI-9564(30 μM, Dox 6 h), P + B(15 μM + 15 μM, Dox 6 h) respectively. Three biological replicates. E Clongenicity of OCT4-GFP positive colony recovered for 48 h in 2i/LIF medium after c-Jun induction with different treatment, DMSO(Dox 0 h) DMSO(Dox 6 h), PFI-3(30 μM, Dox 6 h), BI-9564(30 μM, Dox 6 h), P + B(15 μM + 15 μM, Dox 6 h) respectively. Data are mean ± s.d., two-sided, unpaired t test; n = 3 independent experiments. **P < 0.01, ****P < 0.0001