Fig. 5
From: Both prokaryotes and eukaryotes produce an immune response against plasmids with 5ʹ-GTTTGTT-3ʹ
Core sequences protect prokaryotic cells from plasmid infection and mutation of the core sequences increases plasmid transformation efficiency. Transformation efficiencies of different mutant forms of pEGFP-N1 in E. coli DH5α (A), E. coli BL21 (B), and E. coli FastT1 (C). Transformation efficiencies of different mutant forms of pXT7-myh9a in E. coli DH5α (D) and E. coli FastT1 (E). F Transformation efficiencies of different sized forms of pXT7-myh9a with mutant core sequence in ori. When the core sequence in ori is mutated, the number of clones is reduced for the small size pXT7-myh9a but increased for the large plasmid. Sanger sequencing results showing that the ori sequence of pEGFP-N1 favors survival compared with the 5ʹ-GTTTGTT-3ʹ sequence (G), but the ori sequence of pXT7 my17 confers no survival advantage (H)