Fig. 3
From: Both prokaryotes and eukaryotes produce an immune response against plasmids with 5ʹ-GTTTGTT-3ʹ
The pEGFP-N1 backbone has sequences that can regulate the expression of isg15. A qRT-PCR results showing that pEGFP-N1, pCMV-VSV-G and pLVX-IRES-ZsGreen1 activate the expression of isg15 in 3 hpf embryos. B Mutation of core plasmid sequences reduces isg15 expression. C qRT-PCR results showing that mutation of core sequences of ori, CMV and HSV in pEGFP-N1 reduces activation of isg15 expression. D Mutation of the core sequence of pCMV-VSV-G and pLVX-IRES-ZsGreen1 weakens responses of HEK 293 T cells. E, F Semi-quantitative PCR and qRT-PCR results showing that the methylation level of H3K4me3 in the isg15 promoter region was significantly increased. MW: DL5000 DNA Molecular Weight Marker