From: Macrophages derived from pluripotent stem cells: prospective applications and research gaps
Infectious agent | Reference | Main findings |
---|---|---|
Salmonella typhi, S. typhimurium | Hale et al. [45] | iMphs are phagocytic and up-regulate inflammation-related genes in response to the infection |
P. aeruginosa | Ackermann et al. [46] | Co-administration of P. aeruginosa and human iMphs to immunodeficient mice prevents the development of the infection; iMph administration shortly after the infection with P. aeruginosa (4 h) decreases infection severity |
S. aureus | Hashtchin et al. [98] | Intratracheal injection of iMphs to immunodeficient humanized mice challenged with S aureus (incuding methicillin-resistant strain) reduces S aureus load, decreases granulocytic infiltration and diminishes lung pathology. Transcriptomic analysis: compared to MDMs, iMphs respond to the infection by a more profound upregulation of inflammatory genes early after infection, however the expression normalizes faster than in MDMs |
L. major | O’Kneefe et al. [91] | After the infection, iMphs contain a higher level of intracellular L. major compared to THP-1, mouse and human bone marrow-derived macrophages |
M. tuberculosis | Nenasheva et al. [33] | iMphs phagocyte and restrict Mtb growth in vitro |
Bernard et al. [90] | iMph infection with either virulent Mtb or the attenuated ESX-1-deficient Mtb mutant allowed to identify a role of ESX-1 secretion system of Mtb in the formation of autophagosomes and the subsequent Mtb escape from autophagosomes to the cytosol | |
Haake et al. [47] | iMphs derived from patients with a complete or partial deficiency in IFN-γR2, IFN-γR1 or STAT1 demonstrate a defective upregulation of HLA-DR, CD64, CD38 and CD282 in response to IFNγ, a decreased phosphorylation of STAT1, no-to-little clearance of BCG. Additionally, STAT1-deficient iMphs have a disturbed production of ROS | |
Han et al. [89] | iMphs are suitable to search for new anti-infectious drugs: the screening of a library of 3.716 compounds for their anti-Mtb activity was performed and a novel anti-Mtb compound, 10-DEBC, was identified | |
HIV-1 | van Wilgenburg et al. [27] | iMphs are infectable with HIV-1 |
Vaughan-Jackson et al. [92] | iMphs are infectable with HIV-1 and ZIKV | |
Taylor et al. [96] | CRISPR/Cas9 engineered iMphs with depleted USP18 exhibit: an increased reactivity to IFNI, a prolonged STAT1 and STAT2 phosphorylation; an enhanced expression of IFN‐stimulated genes; an increased restriction of HIV replication | |
ZIKV, DENV | Lang et al. [93] | Differences in iMph response to DENV and ZIKV have been demonstrated: DENV induced a higher inflammatory response, a higher production of MIF and a decreased iMph migration; ZIKV inhibited NF-kB signaling pathway |