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Fig. 4 | Cell & Bioscience

Fig. 4

From: Development of a novel human CD147 knock-in NSG mouse model to test SARS-CoV-2 viral infection

Fig. 4

Increased sensitivity of hCD147KI-NSG and hACE2-NSG mice to SARS-CoV-2 viral infection compared to WT-NSG littermates. A Average body weight loss as a percent of original body weight in WT-NSG, hCD147KIhet-NSG, and hACE2KIhomo-NSG mice following intranasal infection with the TCID50 dose of SARS-CoV-2 virus (1 × 105 PFU in 25 µL per nostril). Error bars represent standard error measure (SEM). B Quantification of total SARS-CoV-2 viral copies in the lungs of infected mice at day 2 post-infection by qRT-PCR, as represented by total N-gene RNA copies per lung lobe (left) and by N-gene log-twofold-change relative to WT-NSG mice (right). Mean values (red) are listed above each group. Each data point represents the average of two duplicate qRT-PCR assays from one mouse lung RNA preparation. C Representative sm-FISH images at 20× (first 3 columns) and 63× (4th column, zoom on region of interest along with Differential Interference Contrast (DIC) overlay) magnifications showing SARS-CoV-2 RNA detection in lung sections from WT (top), hCD147KIhet (middle), and hACE2KIhomo (bottom) mice, 7 days post-infection. Blue: DAPI; green: SARS-CoV-2 RNA. Scale bar represents 50 µm. N = 5 in WT-NSG, n = 3 in hCD147KIhet and n = 4 in hACE2KIhomo mice. Statistical significance was determined using an unpaired one-tailed t test. *indicates significance for comparisons between hACE2KIhomo-NSG and WT-NSG mice and # indicates significance for comparisons between hCD147KIhet-NSG and WT-NSG mice where number of symbols indicates significance strength (*or #, p < 0.05; **or ##, p < 0.01)

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