Fig. 4

Absence of Mxc results in ectopic expression of Dpn in the progeny of NBs. A, B Confocal images of the third-instar larval brains of wt (A) and mxc RNAi (B), labeled by anti-Dpn (red). Compared to the control (A), more Dpn⁺ cells were shown in mxc RNAi central brains (B). Different cell cycle stages may affect the patterns of Dpn in NB nucleus. (C) Statistical analysis of the numbers of Dpn⁺ cells in the larval central brains of wt and mxc RNAi lines. The data are plotted as mean ± SD. ****p < 0.0001 using a Student’s t test, p = 5.709E–07. Numbers of brain lobes counted N = 6, N = 4, respectively. D–E’ Third-instar larval brains containing Type-I NB FLP-out clones of wt (D–D’) and mxc RNAi (E–E’), labeled by anti-Dpn (red) and anti-GFP (green). In the wt clone (D), Dpn was only expressed in the NBs. In the mxc RNAi clone (E), Dpn was detected in the GMCs (arrowheads). Scale bars: 10 μm. F–G’ Type I NB MARCM clones of wt (F–F’) and mxc^{22a − 6} (G–G’) in third-instar larval brains, labeled by anti-Dpn (red) and anti-GFP (green). Dpn (arrowheads) was detected in the GMCs in mxc^{22a − 6} clone (G), but not in wt GMCs (F). Scale bars: 10 μm