Fig. 1.

Knocking down mxc causes neuronal defects. A Schematic of the structure of Drosophila adult brain, including central brain (CB) and optic lobes (OLs). B Statistics on survival rate of wt, mxc RNAi and Mxc rescue flies after eclosion. The data are plotted as mean ± SD. *p < 0.05 using a Student’s t test, p = 0.0124. Numbers of flies counted N = 99, N = 107, N = 106, respectively. C–E Z-axis projection of adult central brains of wt (C), wor > mxc RNAi (D), wor > mxc RNAi, UAS-Mxc (E), labeled by anti-Elav. Elav is a maker of differentiated neurons. Elav⁺ cells were distributed throughout wt central brains (arrowheads; C), but were missing in mxc RNAi central brains (arrowheads; D). Overexpressing Mxc in mxc knockdown background rescued the phenotype (arrowheads; E). Scale bars: 100 μm