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Fig. 5 | Cell & Bioscience

Fig. 5

From: Blockade of the Arid5a/IL-6/STAT3 axis underlies the anti-inflammatory effect of Rbpjl in acute pancreatitis

Fig. 5

Rbpjl downregulates Arid5a expression and thus inhibits the IL-6/STAT3 axis to alleviate inflammatory response in LPS-induced pancreatic acinar cells. A mRNA expression of Rbpjl, Arid5a and IL-6 after MPC-83 cells with oe-Rbpjl or combined with oe-Arid5a (for 72 h) were stimulated by LPS (for 24 h) determined by RT-qPCR. B Western blot assay was used to detect the expression of Rbpjl, Arid5a and intranuclear STAT3 proteins, total protein expression of STAT3 as well as STAT3 phosphorylation level after MPC-83 cells with oe-Rbpjl or combined with oe-Arid5a (for 72 h) were stimulated by LPS (for 24 h). C CCK-8 assay was used to detect the proliferation of MPC-83 cells with oe-Rbpjl or combined with oe-Arid5a (for 72 h) stimulated by LPS (for 24 h). D CCK-8 assay was used to detect the proliferation of MPC-83 cells with oe-Arid5a (for 72 h) or combined with JSI-124 (for 24 h) stimulated by LPS (for 24 h). E Apoptosis of MPC-83 cells with oe-Rbpjl or combined with oe-Arid5a (for 72 h) stimulated by LPS (for 24 h) detected by flow cytometry. F Apoptosis of MPC-83 cells with oe-Arid5a (for 72 h) or combined with JSI-124 (for 24 h) stimulated by LPS (for 24 h) detected by flow cytometry. G Ultraviolet spectrophotometers were used to detect GSH and MDA production in MPC-83 cells with oe-Rbpjl or combined with oe-Arid5a (for 72 h) stimulated by LPS (for 24 h). H Ultraviolet spectrophotometers were used to detect GSH and MDA production in MPC-83 cells with oe-Arid5a (for 72 h) or combined with JSI-124 (for 24 h) stimulated by LPS (for 24 h). I TNF-α, IL-1β and IL-6 expression in MPC-83 cells with oe-Rbpjl or combined with oe-Arid5a (for 72 h) stimulated by LPS (for 24 h). J TNF-α, IL-1β and IL-6 expression in MPC-83 cells with oe-Arid5a (for 72 h) or combined with JSI-124 (for 24 h) stimulated by LPS (for 24 h). * p < 0.05 vs. MPC-83 cells treated with LPS + oe-NC. # p < 0.05 vs. MPC-83 cells treated with LPS + oe-Rbpjl + oe-NC. & p < 0.05 vs. MPC-83 cells treated with LPS + oe-Arid5a + DMSO. The cell experiment was conducted three times independently

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