Fig. 3
Rbpjl down-regulates the expression of Arid5a by binding to its promoter region. A Analysis of the expression of Arid5a in AP samples in the GSE121038 dataset. B The binding site of Rbpjl and the Arid5a promoter region was predicted by the JASPAR website. C Detection of mRNA and protein expression of Arid5a in the pancreatic tissues of AP mice by RT-qPCR and Western blot assay (* p < 0.05 vs. sham-operated mice). D Detection of mRNA and protein expression of Arid5a in the LPS-induced MPC-83 cells at different time points by RT-qPCR and Western blot assay (* p < 0.05 vs. control cells). E Correlation of Arid5a protein expression with Rbpjl protein expression in LPS-induced MPC-83 cells at different time points analyzed by Pearson's correlation coefficient (* p < 0.0001). F The binding of Rbpjl to the Arid5a promoter region was analyzed by ChIP after pancreatic acinar cells with oe-Rbpjl or sh-Rbpjl (for 72 h) were stimulated by LPS (for 24 h) (* p < 0.05 vs. cells treated with sh-NC. # p < 0.05 vs. cells treated with oe-NC). G, Rbpjl protein binding to the Arid5a promoter region in pancreatic acinar cells was analyzed by EMSA. H Luciferase activity of WT-Arid5a promoter and MUT-Arid5a promoter in HEK-293 T cells transfected with oe-Rbpjl was detected by dual luciferase reporter assay (* p < 0.05 vs. HEK-293 T cells transfected with oe-NC). I The mRNA and protein expression of Arid5a was measured by RT-qPCR and Western blot assay after pancreatic acinar cells with oe-Rbpjl or sh-Rbpjl (for 72 h) were stimulated by LPS (for 24 h) (* p < 0.05 vs. cells treated with sh-NC. # p < 0.05 vs. cells treated with oe-NC). The mouse experiments were performed 14 days after AP modeling, with n = 8 for mice in each group. The cell experiment was conducted three times independently