Fig. 2

Overexpression of Rbpjl inhibits LPS-induced inflammatory response in pancreatic acinar cells. Control and LPS-induced MPC-83 cells were treated with oe-Rbpjl or sh-Rbpjl. A The protein expression of Rbpjl in control and LPS-induced MPC-83 cells was detected by Western blot assay. B The control and LPS-induced MPC-83 cell viability was detected by CCK-8 assay. C Apoptosis of control and LPS-induced MPC-83 cells detected by flow cytometry. D GSH and MDA production in control and LPS-induced MPC-83 cells were detected using an ultraviolet spectrophotometer. E The production of ROS in control and LPS-induced MPC-83 cells. F The expression of pro-inflammatory factors in control and LPS-induced MPC-83 cells was detected by Western blot assay. & p < 0.05 vs. control MPC-83 cells + oe-NC. @ p < 0.05 vs. control MPC-83 cells + sh-NC. * p < 0.05 vs. MPC-83 cells treated with LPS + oe-NC. # p < 0.05 vs. MPC-83 cells treated with LPS + sh-NC. Cell experiments were performed after 72 h of lentivirus transduction and 24 h of LPS stimulation. The cell experiment was conducted three times independently