Fig. 1

Rbpjl is under-expressed in both pancreatic tissues of AP mice and LPS-induced pancreatic acinar cells. A A volcano map of the expression of DEGs in AP samples in GSE121038 microarray (red dot indicates highly expressed genes, green dot indicates poorly expressed genes, X axis represents -log10, and Y axis indicates logFC. B Venn map of AP-related genes from GeneCards and CDT databases and differential genes obtained from GEO database. C A heat map of 32 candidate genes involved in the regulation of AP in AP samples (green to red indicates the expression value from small to large). D The expression of Rbpjl in AP samples in the GSE121038 microarray. E Immunohistochemistry analysis of Rbpjl protein in pancreatic tissues of sham-operated or AP mice (* p < 0.05 vs. sham-operated mice). F The apoptosis rate in pancreatic tissues of sham-operated or AP mice was detected by TUNEL staining (* p < 0.05 vs. sham-operated mice). G The expression of pro-inflammatory factors in the serum of sham-operated or AP mice was detected by ELISA (* p < 0.05 vs. sham-operated mice). H, Western blot assay was used to detect the protein expression of Rbpjl in control pancreatic acinar cells or pancreatic acinar cells treated with LPS at different time points (* p < 0.05 vs. control cells). I The expression of pro-inflammatory factors in control pancreatic acinar cells or pancreatic acinar cells treated with LPS for 24 h was detected by Western blot assay. (* p < 0.05 vs. control cells). The mouse experiments were performed 14 days after AP modeling, with n = 8 for mice in each group. The cell experiment was conducted three times independently