Fig. 5
From: Bone morphogenetic protein signaling regulation of AMPK and PI3K in lung cancer cells and C. elegans
AMPK suppress BMP signaling in lung cancer cells. A, B Immunoblot showing long form (LF) and short form (SF) of lung cancer cells in regular media treated with increasing doses of the AMPK inhibitor AICAR for 24 h. AMPK expression was normalized to Actin. C Immunofluorescence imaging for BMPR2 at the cell surface of lung cancer cells treated with AICAR (500 μM) for 24 h. Graph of mean fluorescence intensity, n = 4. D, E Representative immunoblot of ID1 and BMPR2 in lung cancer cells with and without LKB1 expression. D, E quantification of normalized ID1 and BMPR2 expression, n = 3