Fig. 6

Knockdown of FANCC interferes with locomotor recovery by increasing neuronal apoptosis in SCI mice. A tissular apoptosis and neuronal loss were determined by TUNEL assay and IF at 7 dpi in NC-sham, NC-SCI and KD-SCI mice. B Quantitative analysis of TUNEL-positive cells. C Quantitative analysis of NeuN-positive neurons. D Representative images for Nissl staining obtained from longitudinal sections centered around the injured core 1.5 mm at a 7 dpi and b 28 dpi in NC-sham, NC-SCI and KD-SCI mice. Scale bar = 250 μm. E Quantitative analysis of the amounts of survived neurons at 7 and 28 dpi; n = 6. F The BMS score post SCI in NC-sham, NC-SCI and KD-SCI mice. G, H Photos of Swimming at 28 dpi, showing the worse trunk instability and uncoordinated action in SCI mice, and statistical analysis of the LSS over a 28 days period; n = 6. *p < 0.05 vs. NC-sham group, #p < 0.05 vs. NC-SCI group by one-way ANOVA followed by Tukey’s post hoc analysis (*p < 0.05, **p < 0.01, and ***p < 0.001)