Fig. 5

FANCC deficiency aggravates glial scar formation, myelin sheath destruction and axon outgrowth impairment. A Double immunofluorescence labeling of microglia for IBA-1 (green) and astrocytes for GFAP (red) obtained from longitudinal sections centered around the injured core 3 mm at 7 dpi in NC-sham, NC-SCI and KD-SCI mice; Scale bar = 500 μm. B Quantitative analysis of the area of microglia scar at 7dpi; n = 6. C Quantitative analysis of the area of astrocyte scar at 7dpi; n = 6. D Representative images for LFB staining obtained from longitudinal sections centered around the injured core 3 mm at a 7 and b 28 dpi in NC-sham, NC-SCI and KD-SCI mice; Scale bar = 500 μm. E Quantitative analysis of the demyelinated area at 7and 28 dpi; n = 6. F HE staining images of cords centered around the injured core 3 mm obtained at (a) 7 and (b) 28 dpi; Scale bar = 500 μm. G Quantitative analysis of the defected area at 7and 28 dpi; n = 6. H Triple immunofluorescence labeling of microglia for IBA-1 (pink), astrocytes for GFAP (green) and neurons and neurofilaments for NF-200 (red) obtained from longitudinal sections centered around the injured core 3 mm at 28 dpi in NC-sham, NC-SCI and KD-SCI mice; Scale bar = 500 μm. I Quantitative analysis of the area of astrocyte scar at 28 dpi; n = 6. J Quantitative analysis of the area of microglia scar at 28 dpi; n = 6. The error bars represent the SD. *p < 0.05 vs. NC-sham group, #p < 0.05 vs. NC-SCI group by one-way ANOVA followed by Tukey’s post hoc analysis (*p < 0.05, **p < 0.01, and ***p < 0.001)