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Fig. 3 | Cell & Bioscience

Fig. 3

From: FANCC deficiency mediates microglial pyroptosis and secondary neuronal apoptosis in spinal cord contusion

Fig. 3

FANCC reduces microglial inflammation-mediated neuronal apoptosis. A Diagrammatize method of co-culture between primary microglia and neurons to mimic microglial inflammation-induced neuronal apoptosis in vivo. B Western blotting performed for the proteins including Bax and Bcl-2 in primary neurons co-cultured with microglia in NC-PBS, NC-LPS, OE-FANCC-LPS, and KD-FANCC-LPS groups; n = 3. β-actin was used as the control. C Bar graph showing the ratio analysis of Bax/Bcl-2. D Representative immunofluorescence labeling images for cleaved-Caspase-3 (red) and NeuN (green) obtained from primary neurons co-cultured with microglia in NC-PBS, NC-LPS, OE-LPS, and KD-LPS groups for 48 h, respectively. The blue staining indicates the DAPI-stained nuclei. Scale bar = 50 μm. E Quantitative analysis of cleaved-Caspase-3. F neuronal apoptosis determined by TUNEL assay in primary neurons co-cultured with microglia. G Quantitative analysis of TUNEL-positive cells in primary neurons co-cultured with microglia. H Images of FCA with PI and Annexin-V-FITC in primary neurons co-cultured with microglia post LPS treatment for 24 h. I Quantitative analysis of the apoptosis level of neurons; n = 4. The error bars represent the SD. *p < 0.05 vs. NC-PBS group, #p < 0.05 vs. NC-LPS group by one-way ANOVA followed by Tukey's post hoc analysis (*p < 0.05, **p < 0.01, and ***p < 0.001)

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