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Fig. 1 | Cell & Bioscience

Fig. 1

From: FANCC deficiency mediates microglial pyroptosis and secondary neuronal apoptosis in spinal cord contusion

Fig. 1

FANCC deficiency induces microglial pyroptosis by regulating p38-MAPK and inflammasome NLRP3. A Western blotting of FANCC protein levels in the spinal cord at 7 days post-injury; n = 3. B Bar graph showing a quantitative analysis of FANCC expression; n = 3. The error bars represent the SD. *p < 0.05 vs. Sham group by t-test (*p < 0.05, **p < 0.01, and ***p < 0.001). C Western blotting of FANCC in LPS-stimulated primary microglia for 24 h; n = 3. β-actin was used as the control. D Bar graph showing the densitometry analysis of FANCC expression. The error bars represent the SD. *p < 0.05 vs. PBS group by t-test (*p < 0.05, **p < 0.01, and ***p < 0.001). E Representative immunofluorescence labeling images for IBA-1 (green) and FANCC (red) in LPS-activated microglia after transfection with OE-FANCC and KD-FANCC; Scale bar = 50 μm. F Western blotting for p-p38, p38, NLRP3, ASC, GSDMD and GSDMD-N in LPS-activated microglia after transfection with OE-FANCC and KD-FANCC; n = 3. G Bar graph showing the ratio analysis of p-p38/p38. H Densitometric analysis of NLRP3 expression. I Densitometric analysis of ASC expression. J Densitometric analysis of GSDMD expression. K Densitometric analysis of GSDMD-N expression. L, M ELISAs performed for the IL-1β and IL-18 in culture medium obtained at 24 h in LPS-activated microglia after transfection with OE-FANCC and KD-FANCC (n = 5). N Representative flow cytometry performed for the distinction of iNOS+&F4/80+ microglia transfected OE-FANCC and KD-FANCC post 24 h LPS inducement. O Bar graph showing the quantitative analysis of the percent; n = 4. The error bars represent the SD. *p < 0.05 vs. NC-PBS group, #p < 0.05 vs. NC-LPS group by one-way ANOVA followed by Tukey’s post hoc analysis (*p < 0.05, **p < 0.01, and ***p < 0.001). NC-PBS microglia were treated with PBS for control, NC-LPS microglia were treated with LPS for 24 h, OE-LPS microglia were transfected with OE-FANCC before LPS stimulation for 24 h, KD-LPS microglia were transfected with KD-FANCC before LPS stimulation for 24 h

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