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Fig. 4 | Cell & Bioscience

Fig. 4

From: Histone deacetylase 3 facilitates TNFα-mediated NF-κB activation through suppressing CTSB induced RIP1 degradation and is required for host defense against bacterial infection

Fig. 4

Reduced protein level of RIP1 is observed in HDAC3 deficient macrophage. AC Western blot analysis of RIP1 in Neuro-2a cells transfected with CTSB. DG Western blot analysis of RIP1 in control and HDAC3 deificient RAW264.7 and BMDMs. HM Western blot analysis of RIP1 in Vector and Hdac3−/− RAW264.7 stimulated with Leupeptin (25 μM), CA-074 (10 μM) or MG132 (10 μM) for 12 h. NQ Western blot analysis of RIP1 in LysmCre and LysmCreHdac3f/f BMDMs stimulated with Leupeptin (100 μM) or CA-074 (40 μM) for 12 h. R, S Western blot analysis of RIP1 in Vector, Hdac1−/−, Hdac2−/−, Hdac3−/−, Hdac8−/− RAW264.7 cells. Data are representative of three independent experiments and showed as mean ± SEM. *P < 0.05 and **P < 0.01

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