Fig. 4From: Histone deacetylase 3 facilitates TNFα-mediated NF-κB activation through suppressing CTSB induced RIP1 degradation and is required for host defense against bacterial infectionReduced protein level of RIP1 is observed in HDAC3 deficient macrophage. A–C Western blot analysis of RIP1 in Neuro-2a cells transfected with CTSB. D–G Western blot analysis of RIP1 in control and HDAC3 deificient RAW264.7 and BMDMs. H–M Western blot analysis of RIP1 in Vector and Hdac3−/− RAW264.7 stimulated with Leupeptin (25 μM), CA-074 (10 μM) or MG132 (10 μM) for 12 h. N–Q Western blot analysis of RIP1 in LysmCre and LysmCreHdac3f/f BMDMs stimulated with Leupeptin (100 μM) or CA-074 (40 μM) for 12 h. R, S Western blot analysis of RIP1 in Vector, Hdac1−/−, Hdac2−/−, Hdac3−/−, Hdac8−/− RAW264.7 cells. Data are representative of three independent experiments and showed as mean ± SEM. *P < 0.05 and **P < 0.01Back to article page