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Fig. 2 | Cell & Bioscience

Fig. 2

From: Histone deacetylase 3 facilitates TNFα-mediated NF-κB activation through suppressing CTSB induced RIP1 degradation and is required for host defense against bacterial infection

Fig. 2

HDAC3 deficient macrophages have elevated expression of Ctsb. A, B Western blot analysis of CTSB in control and HDAC3 deficient RAW264.7. C, D Confocal micrographs of RAW264.7 cells stained with DAPI (blue, DNA) and antibodies against CTSB (AF488). Scale bar: 100/10 μm. E PCR analysis of Ctsb in LysmCre and LysmCreHdac3f/f BMDMs. F, G Western blot analysis of CTSB in LysmCre and LysmCreHdac3f/f BMDMs. H PCR analysis of Ctsb in Vector, Hdac1−/−, Hdac2−/−, Hdac3−/−, Hdac8−/− RAW264.7 cells. I, J Western blot analysis of HDAC1, HDAC2, HDAC3, HDAC8 and CTSB in Vector, Hdac1−/−, Hdac2−/−, Hdac3−/−, Hdac8−/− RAW264.7 cells. Data are representative of three independent experiments and showed as mean ± SEM. *P < 0.05 and **P < 0.01

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