Fig. 8

BHLHE40 deficiency alleviates bone loss due to estrogen deficiency in vivo. A Representative Micro CT images of femurs from Wt (Sham) Wt (OVX), Bhlhe40^−/− (Sham) and Bhlhe40^−/− (OVX) mice (Scale bars, 1 mm). B Quantification of cortical bone parameters by Micro CT from Wt (Sham) Wt (OVX), Bhlhe40^−/− (Sham) and Bhlhe40^−/− (OVX) mice. BV/TV; BS/TV; Tb. N (Wt (Sham): n = 6, Wt (OVX): n = 5 Bhlhe40^−/− (Sham): n = 7, Bhlhe40^−/− (OVX): n = 5). C H&E staining of femur sections from Wt (Sham) Wt (OVX), Bhlhe40^−/− (Sham) and Bhlhe40^−/− (OVX) mice (Scale bar, 200 μm). D TRAP staining of femur sections from Wt (Sham) Wt (OVX), Bhlhe40^−/− (Sham) and Bhlhe40^−/− (OVX) mice. The yellow arrowhead points to OCs (Scale bar, 100 μm). E Representative Micro CT images of femurs from Wt (2 months) Wt (12 months), Bhlhe40^−/− (2 months) and Bhlhe40^−/− (12 months) mice (Scale bars, 1 mm). F Quantification (n = 5) of cortical bone parameters by Micro CT from Wt (2 months), Wt (12 months), Bhlhe40^−/− (2 months) and Bhlhe40^−/− (12 months) mice. BV/TV; BS/TV; Tb. N; Tb.Pf; SMI. All data are mean ± SD; * P < 0.05, ** P < 0.01, *** P < 0.001. by one-way ANOVA followed by Tukey’s post hoc test