Fig. 7

c-Fos/Nfatc1 is required for Bhlhe40 function in osteoclast differentiation. A, B TRAP staining and quantification (n = 3) to detect osteoclast differentiation of BMMs in Control, Bhlhe40 overexpression, Control treated with Si-Nfatc1, and Bhlhe40 overexpression treated with Si-Nfatc1 groups (Scale bar, 50 μm). C OC-specific gene expression of BMMs in Control, Bhlhe40 overexpression, Control treated with Si-Nfatc1, and Bhlhe40 overexpression treated with Si-Nfatc1 groups (n = 3). D, E TRAP staining and quantification (n = 3) to detect osteoclast differentiation of BMMs in Control, Bhlhe40 overexpression, Control treated with Si-Fos, and Bhlhe40 overexpression treated with Si-Fos groups (Scale bar, 50 μm). F OC-specific gene expression of BMMs in Control, Bhlhe40 overexpression, Control treated with Si-Fos, and Bhlhe40 overexpression treated with Si-Fos groups (n = 3). G, H TRAP staining and quantification (n = 3) to detect osteoclast differentiation of BMMs in Control, Bhlhe40 overexpression, Control treated with NFAT Inhibitor, and Bhlhe40 overexpression treated with NFAT Inhibitor groups (Scale bar, 50 μm). I OC-specific gene expression of BMMs in Control, Bhlhe40 overexpression, Control treated with NFAT Inhibitor, and Bhlhe40 overexpression treated with NFAT Inhibitor groups (n = 3). NFAT Inhibitor reduced the expression of NFATc1. J, K TRAP staining and quantification (n = 3) to detect osteoclast differentiation of BMMs in Control, Bhlhe40 overexpression, Control treated with T-5224, and Bhlhe40 overexpression treated with T-5224 groups (Scale bar, 50 μm). L OC-specific genes expression of BMMs in Control, Bhlhe40 overexpression, Control treated with T-5224, and Bhlhe40 overexpression treated with T-5224 groups (n = 3). T-5224 reduced the expression of c-Fos. All data are mean ± SD; * P < 0.05, ** P < 0.01, *** P < 0.001. by one-way ANOVA followed by Tukey’s post hoc test