Fig. 5

Bhlhe40 regulates c-Fos and NFATc1 in osteoclast differentiation. A Heatmap of differentially expressed genes (DEGs) for BMMs in Wt versus Bhlhe40^−/− mice (n = 6). B Volcano plot of RNA-seq data of BMMs in Wt and Bhlhe40^−/− mice. C GOBubble plot of KEGG enrichment analysis in DEGs. D GOCircle plot of KEGG enrichment analysis of the DEGs. E GOchord of KEGG enrichment analysis of DEGs, including “Osteoclast differentiation”, “Rheumatoid arthritis”, “Hematopoietic cell lineage”, “Hippo signaling pathway”, “NF-κB signaling pathway”, and “Endocrine resistance”. F The predicted result of downstream genes regulated by Bhlhe40. List1 mentioned Bhlhe40 target genes predicted by Gene Tranion Regulation Database. List2 mentioned the DEGs from our RNA-seq. List3 mentioned the key genes for osteoclastogenesis. List4 mentioned significant altered genes during osteoclastogenesis. G RNA expression of predicted genes in BMMs from Wt and Bhlhe40^−/− mice (Nfatc1: n = 6; Fos: n = 6; Src: n = 6; Ocstamp: n = 6; Slc9b2: n = 5; Tnfrsf11b: n = 3; Grp68: n = 6; Grp183: n = 5). H The mRNA expression levels of BHLHE40 target genes in Control and BHLHE40 overexpression BMMs (n = 3). All data are mean ± SD; ns P > 0.05, * P < 0.05, ** P < 0.01, *** P < 0.001. by unpaired Student’s t test