Fig. 2

Bhlhe40 promotes OC differentiation in vitro. A RNA expression of Bhlhe40 in control (Si-NC) and Bhlhe40 knockdown (Si-Bhlhe40) BMMs (n = 3). B, C Quantification (n = 3) and TRAP staining in Si-NC and Si-Bhlhe40 (Scale bar, 50 μm). D Phalloidin staining in Si-NC and Si-Bhlhe40 (Scale bar, 50 μm). E RNA expression of OC-specific genes in Si- NC and Si-Bhlhe40 (n = 3). F RNA expression of Bhlhe40 in control (Control) and Bhlhe40 overexpressing (Bhlhe40) BMMs (n = 3). G, H Quantification (n = 3) and TRAP staining in Control and Bhlhe40 (Scale bar, 50 μm). I Phalloidin staining in Control and Bhlhe40 overexpression (Scale bar, 50 μm). J RNA expression of OC-specific genes in Control and Bhlhe40 overexpression groups (n = 3). All data are mean ± SD; * P < 0.05, ** P < 0.01, *** P < 0.001. by unpaired Student’s t test