Fig. 1

Bhlhe40 upregulation correlates with OC differentiation and osteoporosis in mice. A Heatmap of mRNA in bone marrow macrophages (BMMs) and osteoclasts (OCs) from dataset GSE54779 on GEO. B Statistical of Bhlhe40 expression between BMMs and OCs in Heatmap (n = 3). C RNA expression of Bhlhe40 in BMMs during osteoclast differentiation (n = 3). D Immunofluorescence of BHLHE40 in BMMs during osteoclast differentiation (Scale bar, 50 μm). E Immunofluorescence of BHLHE40 in RAW264.7 during osteoclast differentiation (Scale bar, 50 μm). F Quantitative analysis (0D: n = 3; 6D: n = 4; Others: n = 5) of BHLHE40 in BMMs during osteoclast differentiation in D. G Quantitative analysis (6D: n = 4; Others: n = 5) of BHLHE40 in RAW264.7 during osteoclast differentiation in E. H, I Immunofluorescence and quantitative analysis (n = 3) of BHLHE40 (green) in BMMs between Sham and Osteoporosis mice. BMMs were stained with CD11b (red). Nucleus was stained with DAPI (blue) (Scale bar, 150 μm). J, K Immunofluorescence and quantitative analysis (n = 3) of BHLHE40 (green) in OCs between Sham and Osteoporosis mice. OCs were stained with CTSK (red). Nucleus was stained with DAPI (blue) (Scale bar, 150 μm). All data are mean ± SD; * P < 0.05, ** P < 0.01, *** P < 0.001. by unpaired (B, C, F, G), paired (I, K) Student’s t test and one-way ANOVA followed by Tukey’s post hoc test