Skip to main content
Fig. 5 | Cell & Bioscience

Fig. 5

From: Dual function of SF3B2 on chromatin and RNA to regulate transcription in head and neck squamous cell carcinoma

Fig. 5

SF3B2 promotes SMC1A and CTCF binding to the SF3B2-binding chromatin regions and modulates SMC1A and CTCF activity. A Silver staining of SDS-PAGE gel with SF3B2-TAP complex components. GFP-TAP was used as control. Proteins were identified using MS/MS. Western blotting shows GFP-TAP, SF3B2-TAP, and endogenous SF3B2 protein expression in FaDu cells. The proteins detected in all three independent experiments are shown. B SF3B2-associated proteins detected by GO enrichment analysis in at least two out of three independent experiments. GO enrichment was analyzed using Metascape [40]. C Sequencing tracks of SMC1A and CTCF ChIP-seq signals and SF3B2 and H3 CUT&Tag signals in GFP- and GFP-SF3B2-FaDu cells. D Overlap of peaks among SMC1A, CTCF, and SF3B2. E Cumulative distribution plot of SMC1A binding levels (GFP-SF3B2/GFP) at peaks with or without overlap with SF3B2 peaks. SMC1A peaks overlapping with SF3B2 peaks, n = 1389. SMC1A peaks not overlapping with SF3B2 peaks, n = 43,573. F Cumulative distribution plot of CTCF binding levels (GFP-SF3B2/GFP) at peaks with or without SF3B2 overlaps. CTCF peaks overlapping with SF3B2 peaks, n = 617. CTCF peaks not overlapping with SF3B2 peaks, n = 24,237. G Box plots showing expression of the nascent transcripts and genes with SMC1A and SF3B2 peaks, or with only SMC1A peaks in SF3B2-depleted or SF3B2-overexpressing FaDu cells. Data expressed as fold change in expression compared to respective controls. Genes with SMC1A and SF3B2 peaks, n = 948. Genes with only SMC1A peaks, n = 7787. H Box plots showing expression of the nascent transcripts and genes with CTCF and SF3B2 peaks, or with only CTCF peaks in SF3B2-depleted or SF3B2-overexpressing FaDu cells. Data is expressed as fold change in expression compared to respective controls. Genes with CTCF and SF3B2 peaks, n = 423. Genes with only CTCF peaks, n = 6045

Back to article page