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Fig. 2 | Cell & Bioscience

Fig. 2

From: Dual function of SF3B2 on chromatin and RNA to regulate transcription in head and neck squamous cell carcinoma

Fig. 2

SF3B2 interacts with gene regulatory elements and RNA. A, B Dot plot of Gene set enrichment analysis between GFP- and GFP-SF3B2-FaDu cells A or between siControl and siSF3B2-treated FaDu cells B. The top five gene ontologies (GO) enriched in the activated or suppressed genes were presented. C Microscopic images of GFP- and GFP-SF3B2-FaDu cells. D Sequencing tracks of H3 and SF3B2 CUT&Tag in FaDu cells, SF3B2 PAR-CLIP in SF3B2-tandem affinity purification (TAP) tag stably-expressing FaDu cells, RNA-seq in GFP- and GFP-SF3B2-FaDu cells, and RNA-seq in siControl- and siSF3B2-treated FaDu cells. E Pie chart of 4459 peaks of SF3B2-binding genomic regions. F Pie chart of 4240 peaks of SF3B2-binding RNA regions. G Overlap of gene number between SF3B2-DNA binding genes and SF3B2–RNA binding genes. H–I Metagene plot of SF3B2 and H3 CUT&Tag peaks H or SF3B2 PAR-CLIP peaks I. TSS, transcription start site; TTS, transcription termination site. J–K The intron and exon junction-centered plots of SF3B2 and H3 CUT&Tag signals J or SF3B2 PAR-CLIP signals K. 5′ss, 5′ splice site. 3′ss, 3′ splice site. L Representative motifs in SF3B2 CUT&Tag peaks. The motif was detected in 16.17% of SF3B2 peaks. M Representative motifs in SF3B2 PAR-CLIP peaks. The motif was detected in 29% of the SF3B2-binding RNA region

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