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Fig. 6 | Cell & Bioscience

Fig. 6

From: Inclusion of a dual signal sequence enhances the immunogenicity of a novel viral vectored vaccine against the capsular group B meningococcus

Fig. 6

Antigen-specific T cell responses induced in mice two weeks after immunisation with human adenovirus vectors encoding bacterial antigens with or without an N-terminal signal sequence. Groups of six BALB/c mice were immunised with human adenovirus serotype 5 (AdHu5) vaccines expressing one of a series of bacterial antigens, with or without an N-terminal signal sequence (SS). Spleens were harvested two weeks post-immunisation, processed, and stimulated at a concentration of 3 μg/mL with the relevant peptide pool. An interferon (IFN)-γ/interleukin (IL)-17A dual colour fluorospot was performed to assess antigen-specific T cell responses associated with these cytokines. A IFN-γ and B IL-17A spot-forming units (SFU) per million cells were quantified for each antigen. Inclusion of an N-terminal SS was found to boost both types of antigen-specific T cell responses. Statistical comparisons were made using a Mann–Whitney U-test. *p < 0.05

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