Fig. 4

Expression of factor H binding protein N-terminal signal sequence variants from HeLa cells infected with adenovirus vectors over the course of 14 h. N-terminal signal sequence variant transgenes were designed to include an enhanced green fluorescent protein sequence and cloned into adenovirus vectors. Green fluorescent protein intensity values were calculated from images taken across three separate fields of the infected wells and averaged for each signal sequence variant. Loess smoothing was applied to trend lines for each construct and 95% confidence intervals are shaded