Fig. 3

Confocal images of HeLa cells infected overnight human adenovirus encoding factor H binding protein (fHbp) N-terminal signal sequence (SS) variants. N-terminal signal sequence variant transgenes were designed to include an enhanced green fluorescent protein (eGFP) sequence and cloned into adenovirus vectors. Representative images for A Uninfected cells (negative control), B eGFP only (positive control), C Full-length SS fHbp 1.1-eGFP, D LTA knockout (KO) SS fHbp 1.1-eGFP, E Signal peptide (SP) KO SS fHbp 1.1-eGFP and F SP + LTA KO SS fHbp 1.1-eGFP. Blue fluorescence indicates DAPI-stained nuclei, green fluorescence indicates antigen-eGFP expression. 16 μm scale bars are shown in the bottom right corner of each image