Fig. 7
From: Hepatitis, testicular degeneration, and ataxia in DIDO3-deficient mice with altered mRNA processing
Altered Pick1 mRNA processing in E16 tissues. a Coverage and alignment of RNA-seq reads from representative Dido1 WT and E16 livers to the mm10 reference genome at the Pick1 locus. The upper frame in each plot depicts coverage; blue tracks in the lower frames represent individual junctions. Note RNA transcription downstream of the last Pick1 exon through the Slc16a8 + Lgals1-ps2 loci in E16. b Detailed view of the coverage and splice junctions at the 3' end of Pick1 and across Lgals1-ps2, and scheme of the oligonucleotide primer pairs designed to assess normal (N) vs. abnormal (A) Pick1 mRNA splicing. c Ethidium bromide-stained agarose gel showing products of the PCR analysis of cDNA from representative Dido1 WT vs. E16 livers with the primer pairs depicted in b. To render this analysis semiquantitative, the PCR was run independently for 33 and 40 cycles. d Plot of ΔCtA-N values from qPCR analysis of mRNA splicing at the Pick1 locus on cDNA samples from three tissues, each from three individual Dido1 WT vs. E16 mice. Filled symbols represent lower ΔCtA-N limits in cases in which abnormal splicing was not detected. Bars are mean ΔCtA-N for each group; an unpaired t-test showed significant difference ( p < 0.05) between E16 and WT in all three organs