Fig. 6

Hallmark pathways in E16 livers. The GSEA program was used to identify gene ontology terms enriched or depleted in the RNA-seq dataset for genes differentially expressed in E16 vs. WT livers. Examples of terms with relevance to the mutant phenotype are plotted. X-axes represent relative expression level of genes in the ontology group (red, increased expression in E16; blue, decreased expression in E16). Y-axes represent gene group enrichment (> 0) or depletion (< 0) in E16. a Gene ontologies preferentially depleted in E16 samples include typical liver-related pathways such as Bile Acid and Cholesterol metabolism. b Gene ontologies preferentially enriched include terms such as IFN-α response and Allograft Rejection, consistent with liver inflammation in E16 mice