Fig. 1

Deletion of the floxed exon and growth arrest in E16 mice. a Ethidium bromide-stained agarose gel showing the products of PCR analysis of genomic DNA from livers of representative Dido1 wt/wt or floxE16/ΔE16 mice, untreated or tamoxifen-treated, with a combination of primers capable of amplifying all three Dido1 alleles of interest. Results were similar for DNA from other tissues. b Variation in body weight over time for individual mice, plotted for each Dido1 genotype. WT n = 11; E16 n = 21. Not all mice were weighed at every time point. Thick grey lines connect mean values. c Early time course of alanine aminotransferase activity in serum samples from Dido1 WT (n = 12) and E16 (n = 12) mice. Each mouse was bled only once. Individual values are shown, as well as dashed lines connecting mean values. Aspartate aminotransferase activity followed a similar course. d Liver-to-body weight ratios for individual Dido1 WT (n = 5) and E16 (n = 7) mice at 40–50 days post-tamoxifen. Bars = mean ratios; an unpaired t-test showed significant difference (p < 0.05)