Fig. 5

Regulation of ERK1/2 pathway by ATF2 is associated with p53. A Differential expressing genes between control and ATF2 overexpressing group in HCT-116 p53+/+ or HCT-116 p53−/− cell were identified with R studio, then gene oncology (GO) enrichment of elevated genes (foldchange > = 2) was analyzed with Metascape, and top GO biological processes were displayed. B Cluster analysis was performed using R studio and genes related with MAPK pathway were marked in the heatmap. C Relative expression of CSK and NF1 in cisplatin treated HCT-116 cells were measured with Real-time quantitative PCR assay. D Protein expression of total ERK1/2 and p-ERK1/2 (Thr202/Tyr204, activated) were detected with Western blot assay. E Real-time quantitative PCR assay was performed to evaluate the expression of CSK and NF1 in cisplatin treated GC cells. F Protein expression of total ERK1/2 and p-ERK1/2 (Thr202/Tyr204, activated) in GC cells were detected with Western blot assay