Fig. 4

ATF2 interacts with the p53 TAD domain. A The co-localization of ATF2 (green) and p53 (red) GC cells was analyzed by confocal imaging. DAPI stains nuclear DNA (blue). The photomicrographs were taken at the original magnification 400 ×. B Molecular docking with the crystal structures of ATF2 and p53 were performed by Discovery Studio software. C The interaction between endogenous ATF2 and p53 was assessed with immunoprecipitation (IP) using ATF2 antibody. D Flag-p53 and Myc-ATF2 plasmids were co-transfected into cancer cells, then protein interaction was confirmed with immunoprecipitation (IP) assay. E Flag-p53-dTAD and Myc-ATF2 plasmids were co-transfected and an IP assay was performed. F The interaction between ATF2 and flag-p53 in vitro was elucidated using a GST-pull down assay. G The sub-cellular localization of ATF2 (green) in cisplatin treated GC cell lines was determined using immunofluorescence (400×). The positive band was marked with black star (*), and the heavy chain of antibody in IP was marked with yellow star (*)