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Fig. 4 | Cell & Bioscience

Fig. 4

From: Integrating spatial transcriptomics with single-cell transcriptomics reveals a spatiotemporal gene landscape of the human developing kidney

Fig. 4

Heterogeneity analysis of the three DTLH clusters located in different renal medullary regions. a The spatial location of the SSBpr/ErprT and SSBmd/DTLH clusters. The green color represents the SSBpr/ErprT cluster (outer layer of the medulla), the red color indicates the SSBmd/DTLHa cluster (the middle layer of the medulla), and the blue color denotes to SSBmd/DTLHb cluster (the inner layer of the medulla); b Heatmap illustrating the genes encoding complex subunits of mitochondrial OXPHOS, gradually increasing from outer medulla to inner medulla; c Expression changes of the genes related to osmotic stress (FXYD2, TonEBP/OREBP, and MUPP1/CLDN4), gradually increasing from outer medulla to inner medulla. The black line represents the variation trend of the average gene expression. P values represent the overall comparison between the three groups using ANOVA. A P-value < 0.05 is statistically significant. d Spatial distribution of FXYD2, TonEBP/OREBP, and CLDN4; e Spatial image depicting the expression of GPX3 in the outer medulla. Scale bar: the white to red gradient corresponds to the gene upregulation levels. f Space projection of scRNA-identified interstitial cells. Scale bar: the blue to red gradient corresponds to the matching degree between cell- type-specific (scRNA-seq) and region-specific (ST-seq) gene sets; g Space projection of scRNA-identified immune cells

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