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Table 1 Primary and secondary antibodies used for all experiments. 

From: Fibronectin extra domain A (FN-EDA) causes glaucomatous trabecular meshwork, retina, and optic nerve damage in mice

Antibody

Raised in Species

Company, Catalogue#

Application

Dilution

Primary antibodies

 TLR4

Rabbit

Proteintech, #19811-1AP

WB

1/1000

 Iba-1

Rabbit

Wako, #019-19741

IHC

1/1000

 Fibronectin

Rabbit

Millipore, AB1945

IHC

1/500

 GAPDH

Mouse

Cell Signalling, #97166

WB

1/5000

 NFH

Mouse

Biolegend, #801701

IHC

1/500

 NFH

Mouse

Biolegend, #835503

IHC

1/500

 GFAP

Chicken

AVES, #AVES-GFAP

IHC

1/500

 RBPMS

Guinea Pig

Phosphosolutions, #1832RBPMS

IHC

1/200

Secondary antibodies

 Anti-Chicken 680

Donkey

LI-COR, 925-68075

WB

1/10000

 Anti-Mouse 800

Goat

LI-COR, 827-08364

WB

1/10000

 Anti-Rabbit 800

Goat

LI-COR, 925-68071

WB

1/10000

 Anti-Mouse 680

Goat

LI-COR, 926-68170

WB

1/10000

 Anti-Rabbit Cy2

Donkey

Jackson Immunoresearch, #711225152

IHC

1/500

 Anti-Mouse Cy2

Donkey

Jackson Immunoresearch, #715225150

IHC

1/500

 Anti-Rabbit Cy2

Donkey

Jackson Immunoresearch, #71175152

IHC

1/500

 Anti-Guinea Pig Cy3

Donkey

Jackson Immunoresearch #706165148

IHC

1/500