Fig. 7

B6.EDA^+/+ mice have increased Iba1 expression in the ONH. (A-H) immunohistochemistry staining for microglia (purple) by Iba1 antibody labeling (A, B), RGC axons (green) by NF200 antibody labeling (C, D), and astrocytes (blue) by GFAP antibody labeling (E, F) in the ONH of C57BL/6J and B6.EDA^+/+ mice at 8–9 months of age. A, B, I B6.EDA^+/+ mice have increased area covered by Iba-1 positive expression in the ONH compared to C57BL/6J controls. C, D, J No significant difference detected in coverage by NF200 labeled ganglion cell axons. E, F, K No significant difference detected in coverage by GFAP labeled astrocytes. Significance determined by Student’s t-test. Scale bar = 50 μm. n = 3 mice/strain