Fig. 6

B6.EDA^+/+ mice have elevated IOP, RGC loss, and ON damage. A IOP is significantly elevated in B6.EDA^+/+ mice (n = 16) compared to C57BL/6J (n = 22) at 12 months of age, mean ± SEM. Significance determined by Student’s t-test. B B6.EDA^+/+ mice (n = 10) have 9.7% loss of RGCs at 12 months of age compared to C57BL/6J (n = 15) as quantified by RBPMS stained retinal flat mounts. Significance determined by Student’s t-test (C) Percent of axons with no damage [1] or with mild damage (2) in transverse sections of optic nerves in B6.EDA^+/+ (n = 23) and C57BL/6J (n = 18) mice. B6.EDA^+/+ have significantly more ON damage compared to C57BL/6J, Significance determined by Fisher's exact test. D Images of representative transverse sections of optic nerves of B6.EDA^+/+ and C57BL/6J mice. * = p < 0.05, **** = p < 0.0001