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Fig. 5 | Cell & Bioscience

Fig. 5

From: Fibronectin extra domain A (FN-EDA) causes glaucomatous trabecular meshwork, retina, and optic nerve damage in mice

Fig. 5

B6.EDA+/+ mice have increased expression of Tlr4 and Iba1 in the TM. A, B Merged fluorescent and brightfield images show increased expression of Tlr4 mRNA by in situ hybridization and Iba1 by immunofluorescent labeling in the TM of B6.EDA+/+ mice (B) compared to controls (A). Unmerged images shown for C57BL/6J (C) and B6.EDA+/+ (D). Puncta of Tlr4 mRNA is red, Iba-1 positive macrophages are green, blue is DAPI nuclear stain. Scale = 100 µm. E Total Tlr4 mRNA puncta in TM of C57BL/6J and B6.EDA+/+ mice quantified by ImageJ analysis. D Quantified coverage of TM area by Iba-1 positive expression in C57BL/6J and B6.EDA+/+ mice. Significance determined by Student’s t-test, *p < 0.05, n = 3 mice/strain

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