Fig. 4

B6.EDA^+/+ mice have smaller giant vacuoles. A, B Fragment of the stitched image of the entire length of SC of C57BL/6J (A) and B6.EDA^+/+ (B) mice. C Quantified percent of giant vacuoles with various sizes from C57BL/6J and B6.EDA^+/+ mice. B6.EDA^+/+ mice had significantly more smaller giant vacuoles. D Total number of giant vacuoles per 100 μm². Significance determined by Chi-square, p < 0.05, n = 3 mice/strain, two planes of stitched sections along the entire length of SC analyzed per mouse, scale bar = 2 µm