Fig. 5

Analysis of the downstream targets of FTO in ECCs. A Volcano map of the gene expression from UID mRNA-seq data between the negative control and FTO overexpression groups; The abscissa: log2 (fold change); the ordinate: − log10 (p-value); Gray dots represent the genes that were not differentially expressed; the blue dots represented the genes that were differentially down-regulated, and the red dots represented the genes that were differentially up-regulated; B Venn diagram of the peak associated genes of meRIP-seq data between the negative control and FTO overexpression groups; C The distribution of the peaks in each functional area of the gene; D Top consensus motif identified by HOMER with meRIP-seq peaks in ECCs with or without FTO overexpression; E Four-quadrant diagram of the meRIP-seq differential genes and mRNA-seq differential genes; F GO Analysis for the genes with reduced methylation and mRNA levels. G Schematic diagram of the screening of downstream target genes; H RNA Immunoprecipitation assay was used to detect the binding between HSD17B11 and FTO protein in KYSE510 cells (The upper band is the targeted band, and the lower band is the antibody heavy chain), and statistical results.