Fig. 4

The FTO expression level influences the tumorigenesis of ECCs in vivo. A Representative images of subcutaneous tumors in immunodeficient Balb/c nude mice with shNC and shFTO ECCs; B Statistical results of the tumor volumes among the three groups; C Survival analysis of mice subcutaneously injected with the ECCs with or without FTO knockdown (x²=18.528,P < 0.001);D Immunohistochemical detection of the FTO expression levels in subcutaneous tumors with or without FTO knockdown and statistical results; E Representative images of the subcutaneous tumors in immunodeficient Balb/c nude mice with FTO OE and vector ECCs; F Statistical results of the tumor volumes between the negative control and FTO overexpression groups; G Survival analysis of the mice subcutaneously injected with the esophageal cancer cell lines with FTO OE and vector (x²=12.429, P < 0.001); H Immunohistochemical detection of the FTO expression levels in the subcutaneous tumors derived from the injection of the negative control and FTO overexpression into mice and statistical results; *P < 0.05, **P < 0.01, ***P < 0.001.