Fig. 3

FTO overexpression promotes the proliferation, migration, and stemness of ECCs. A CCK-8 assay was used to detect cell proliferation of ECCs in the negative control and FTO overexpression groups; B Cell colony formation assays were used to detect the long-term proliferation of ECCs in the negative control and FTO overexpression groups; C Cell migration results of wound-healing assays, and the D Transwell assay in the negative control and FTO overexpression groups and the respective statistical results; E Using extreme limiting dilution assay analysis, the FTO overexpression group was shown to have an increased spheroidization ability compared to the control group; the frequency of stem cells in each group was estimated using 95% confidence intervals, and the difference between the two groups was analyzed using chi-square test; F Sphere formation ability of FTO OE and vector ECCs, the histograms on the right represent the statistical results. ^*P < 0.05,^**P < 0.01, ^***P < 0.001.