Fig. 2

FTO inhibition decreases the proliferation, migration, and stemness of ECCs. A CCK-8 assay was used to detect the cell proliferation in ECCs with or without FTO knockdown; B Cell colony formation was used to detect the long-term proliferation of ECCs with or without FTO knockdown; C Cell migration results of wound-healing assays, and the D Transwell assay performed using ECCs with or without FTO knockdown and the respective statistical results; E Using extreme limiting dilution assay analysis, the frequency of stem cells in each group was estimated with 95% confidence intervals, and the differences among the groups were analyzed using chi-square test; F Sphere formation ability of shFTO and shNC ECCs, the histograms on the right represent the statistical results. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001.