Fig. 1
FTO is elevated in esophageal cancer stem-like cells and is a poor prognostic factor in EC patients. A EpiQuik m6A RNA Methylation Quantification kit (Colorimetry) was used to detect the total methylation levels of mRNA extracted from normal or sphere cells; B qPCR analysis of ALKBH5 and FTO mRNA expression levels between normal and sphere cells; C Immunohistochemical analysis of FTO protein in paracancerous and cancer tissues of EC patients; D Statistical results of the FTO protein levels in paracancerous and cancer tissues of EC patients (t=11.27, P < 0.001); E Kaplan-Meier survival curve was used to analyze the overall patient survival in groups with a high and low FTO expression. Log-rank test was used to compare the median overall survival duration between the two groups (x2=15.233, P < 0.001).