Fig. 4

Molecular features of immunocyte subclusters of the human amnion. A The t-SNE plot displays six subclusters of immunocytes. B Dot plots show the top DEGs in each subset of immunocytes. C Feature plots of macrophage markers (CD68, CD86, CD163 and MRC1). D The t-SNE plot displays fetal or maternal origin of immunocytes. E Proportions of macrophages of fetal or maternal origin in TNL_P, TL_P and TL_D groups. F GO analysis of DEGs in subclusters 3 and 6. G Overlaying the grouping information on the t-SNE plot of immunocytes. H Comparison of the proportions of subclusters 1, 4 and the combined proportion of subclusters 3 and 6 in TNL_P, TL_P and TL_D groups. *P < 0.05; ***P < 0.001. Unpaired Student’s t-test was used to assess the difference between TNL_P and TL_P, and paired Student’s t-test was used to assess the difference between TL_P and TL_D. TNL_P term non-labor-proximal, TL_P term labor-proximal (ZAM zone), TL_D term labor-distal (non-ZAM zone)